Hordein signal genes of certified Czech and Slovak varieties of barley (Hordeum vulgare)
1995
Sasek, A. | Bradova, J. (Vyzkumny Ustav Rostlinne Vyroby, Prague-Ruzyne (Czech Republic)) | Cerny, J.
Model electrophoretic hordein spectra of 25 certified varieties of spring barley and 10 certified varieties of winter barley have been compiled. Twenty-five grains of each variety were separately assessed by the method of hordein vertical electrophoresis in columns of starch gel with Al-lactate buffer. Hordein allelic blocks were singled out of the electrophoretic spectra of hordeins and they were characterized by the number of zones, their relative mobility (REM) and staining intensity. The catalogue of hordein electrophoretic spectra and/or catalogue of singled out sets of allelic hordein blocks of zones enable to identify the varieties in a grain sample of seed and mercantile lots.
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