Direct gene transfer to protoplasts [Nicotiana tabacum; N. plumbaginifolia; Medicago sativa; Triticum monococcum]
1990
Larkin, P.J. | Taylor, B.H. | Gersmann, M. | Brettell, R.I.S. (Commonwealth Scientific and Industrial Research Organization, Canberra (Australia). Div. of Plant Industry)
Direct transfer of genes to protoplasts is considered, using electroporation and polyethylene-glycol-induced plasmid uptake. Both transient expression and stable transformation are discussed, with data and comparisons from the following protoplast systems: Nicotiana plumbaginifolia suspension culture, Nicotiana tabacum leaf, Medicago sativa leaf and Triticum monococcum suspension culture.
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