Effect of the rolABC, rolAB and CaMV 35S-rolC genes on growth and nitrogen fixation in Lotus corniculatus
1995
Pozarkova, D. | Siffelova, G. | Nasinec, V. (Akademie Ved, Ceske Budejovice (Czech Republic). Ustav Molekularni Biologie Rostlin) | Machackova, I.
Lotus corniculatus has been transformed by Agrobacterium tumefaciens harbouring the rolABC, rolAB, and 35S-rolC constructs. Growth, shoot and root morphology, cytokinin and auxin concentrations in the shoots, chlorophyll content in the leaves, nodulation and nodule nitrogenase activity of clonal progenies of selected transformants, in which the transferred rol sequences were proved, were compared with control plants. The most striking alterations were observed in the 35S-rolC transformants in comparison with either the rolAB, rol ABC, or controls. The rolAB and rolABC transformants were very similar to the control plants in their morphology as well as in most other traits measured.
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