Detached leaf assay from screening chickpea for resistance to ascochyta blight
1994
Dolar, F.S. (University of Toronto, Toronto, Ontario.) | Tenuta, A. | Higgins, V.J.
The feasibility of using a detached leaflet assay for disease assessment of ascochyta blight on chickpea was examined by comparing disease severity values for inoculated plants with disease severity and disease incidence values for inoculated detached leaflets floating on water in petri plates. Three isolates of Ascochyta rabiei, collected in Turkey, and representing races 1, 4, and 6, were tested on six chickpea cultivars. For young detached leaflets (most recent fully expanded leaves), there was a high positive correlation of disease severity, or disease incidence, values with disease severity values for intact plants. The resistant or susceptible category for each cultivar in relation to each race was not changed by assay on young detached leaflets, but for older leaflets (from the first or second oldest leaves) a susceptible rating sometimes was altered to a resistant rating
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