Long-term in vitro maintenance of oil palm (Elaeis guineensis Jacq.) clones through axenic root cultures
1995
Rohani, O. | Paranjothy, K. (Palm Oil Research Institute of Malaysia, Bandar Baru Bangi, Selangor (Malaysia))
Studies were carried out to determine whether oil palm clones could be maintained long-term in vitro by means of root cultures. It was found that isolated root explants excised from axenic ramets could be maintained in culture for at least 18 months by repeated subculture. Root explants 3 cm in length produced more axillary roots than 1 cm root explants. Root growth was best in basal liquid medium containing modified MS nutrients supplemented with 1.5 g/L of activated charcoal. A full strength modified MS medium supported root tip elongation and production of axillary roots better than a half strength nutrient medium
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