Detection of antibacterial activity of commertial lactic acid starter cultures
1995
Rogelj, I. | Bogovic-Matijasic, B. (Ljubljana Univ. (Slovenia). Biotechnical Fac., Zootechnical Dept.) | Canzek, A. (Ljubljana Univ. (Slovenia). Biotechnical Fac., Food Science and Technology Dept.)
21 commercial starter cultures were tested for their antibacterial activity against 24 indicator bacteria. Specter of antimicrobial activity and probable presence of bacteriocin-like substances were detected with direct agar spot method. 13 of 21 tested commercial starter cultures have shown antibacterial activity against indicator bacteria, while the production of bacteriocin-like substance was found with starter cultures MSK B2, LA-CH-5, L. acid. 145 and MSK/V10. In cell-free supernatant no bacteriocin-like activity was detected. Strains of starter cultures BB 12, L. casei 01, LA-CH-5, L. casei R, MSK/V10 (L. acidophilus) were resistant at pH 2-5 and bile salts concentration 0,1% - 1,5%. The most promising as protective cultures or dietary adjuncts are L.casei 01, BB12, LA-CH-5, L.casei R, L.acid 145, MSK B2 and MSK/V10.
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