The evaluation of serology and bacteriology for the diagnosis of bovine brucellosis in South Sulawesi
1993
Sulaiman, I. | Patten, B.E. | Darmadi, P. (Balai Penyidikan Penyakit Hewan Wilayah VII, Maros (Indonesia))
English. Serum plus the supramammary, prefemoral, prescapular, retopharyngeal and mesenteric lymph nodes were obtained from 627 cattle slaughtered at the city abattoirs. The sera were tested by the Rose Bengal Plate Test (RPBT), Complement Fixation Test (CFT) and Enzyme-linked Immunosorbent Assay (ELISA), and lymph nodes were examined bacteriologically. A total of 93 sera were positive in one of the serological tests and 68 B. abortus isolates were obtained from the lymph nodes from these positive cattle. Lymph nodes from 83 cattle negative in all of the serological tests were examined bacteriological and 5 B. abortus isolates were obtained. Four of the 5 isolates from the negative cattle were typed as Strain 19 whereas only 13 of the 68 isolates from serologically positive cattle were Strain 19 isolates
Show more [+] Less [-]unknown. Serum sapi dan limfoglandulla diambil dari 627 sapi yang dipotong di Rumah Potong Hewan. Uji Rose Bengal (RBPT), Uji pengikatan komplemen (CFT) dan Uji Enzyme-linked Immunosorbent Assay (ELISA) digunakan untuk pengujian serum. Sedangkan limfoglandulla yaitu supramammaria, prefemoralis, prescapularis, retrofaringealis, dan mesenterialis diperiksa secara bakteriologis. Limfoglandulla dari 93 ekor sapi yang menunjukkan positif serologis dan limfoglandulla dari 83 ekor sapi yang menunjukkan negatif serologis dikultur untuk pemeriksaan bakteriologi. 68 dari 93 ekor positif serologis diisolasi B. abortus, empat diantaranya diidentifikasi B. abortus strain 19
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