Long-term storage and in vitro propagation of Lolium perenne
1992
Schmidt, F. | Posselt, U.K. (Zentralstelle fuer Agrardokumentation und -information, Bonn- Bad Godesberg (Germany). Informations- und Koordinierungszentrum fuer Genetische Ressourcen)
Lateral buds of six Lolium perenne inbred lines 5 to 15 mm long were cultured on MS-medium supplemented with 0.2 mg 1-(1) kinetin. After four to six weeks the plantlets were well developed and could be stored in a cold room at a temperature of 2 degrees C to 4 degrees C up to 18 month before subculture was neccessary. For multiplication these plantlets were transferred to MS-media containing cytokinins in various concentrations and combinations. After five weeks the induced tillers were counted, separated, transferred to soil and grown in a greenhouse for six weeks. Tiller number, fresh and dry matter yield of the plants were determined and the dry matter content was calculated.
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