Determination of toxigenic Fusarium spp. in the domestic wheat - using the ICFM methodological recommendation
1998
Ostry, V. | Ruprich, J. | Kozisek, J. (Statni Zdravotni Ustav, Brno (Czech Republic). Narodni Referencni Centrum pro Mikroskopicke Houby a jejich Toxiny v Potravinovych Retezcich)
Fifty one food wheat samples from three production regions in the Czech Republic have been mycologically examined. Fusarium species were isolated by the method of grain rinse with sterile 0.1 % pepton in water and by the method of direct plating of grains after their surface sterilization: Czapek Dox Iprodione Dichlorane Agar (CZID) was used for cultivation. The identification of the isolated strains has been done according to Nelson et al. and by comparing with collection strains of the genus Fusarium. Fusarium spp. isolated from food wheat samples showed the greatest frequency in the following order: Fusarium graminearum, F. avenaceum, F. sporntrichioides, F. reticulatum and F. solani.
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