Morphology and developmental potential of bovine parthenotes after spontaneous activation in vitro
1998
Lechniak, D. | Cieslak, D. | Sosnowski, J. (Agricultural University, Poznan (Poland). Dept. of Genetics and Animal Breeding)
Altogether, 1403 folicular oocytes, collected by the aspiration method, were matured in vitro and then cultured without insemination in the same conditions as a group of inseminated oocytes. After 48-72 h of additional culture, 141 oocytes (10 percent) were found to be spontaneously activated. Morphological evaluation revealed that the number of blastomeres within parthenotes ranged from 2 to 16 cells, with minority comprising 9-16 blastomeres. According to a cytogenetic analysis, only 1.2 percent of the analysed parthenotes consisted of more than 9 cells. Parthenotes may not be distinguished from embryo produced in vitro and spontaneous parthenogenetic activation and in vitro maturation and fertilization system indicates suboptimal culture conditions
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