Methodical aspects of hop (Humulus lupulus) genetic transformation
1999
Oriniakova, P. (Jihoceska Univ., Ceske Budejovice (Czech Republic). Prirodovedecka Fakulta) | Pavingerova, D. | Matousek, J.
The main goal of this work was to optimize the standard protocol used for hop transformation in respect to the most usual reasons of low frequency of transgenic plant regeneration. Slightly higher frequency (40%) of transformation was achieved only using pre-cultivation of hop explant on regeneration media before transformation in comparison with the standard method (36%) . The use of acetosyringone, a vir genes inducing phenolic signal molecule, during A. tumefaciens cultivation, did not increase frequency of callogenesis on selective regeneration media. The callogenesis and 5-azacytidine did not affect positively the ability of shoots to root on selective media. The use of antioxidants polyvinylpolypyrrolidone (PVPP) and dithiotreitol (DTT) did not affect the result of hop transformation.
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