Use of immunomagnetic separation in detection of Salmonella and Listeria from foodstuffs
1999
Karpiskova, R. | Holasova, M. (Statni Zdravotni Ustav, Brno (Czech Republic). Centrum Hygieny Potravinovych Retezcu)
Immunomagnetic separation (IMS) and conventional methods (ISO guidelines) were used for the routine testing of various foodstuffs for the presence of Salmonella spp. and Listeria spp. The total of 408 food samples were examined for the presence of Salmonella spp. and 33 samples (8 %) were positive using conventional methods. Using IMS 49 (12 %) of them were positive. The sensitivity of IMS was higher than in conventional methods. The statistical differences were of great significance especially when using Rappaport Vassiliadis medium. The number of 375 food samples were examined for the presence of Listeria spp., 81 samples (21.6 %) were positive using conventional methods and 91 (24.3 %) were positive using IMS. The statistical differences were significant when using the USDA method and there were no significant differences between IMS and FDA method. Our results demonstrated that the IMS technique was either more sensitive than or as sensitive as the conventional detection procedures and saved at least one day eliminating the selective enrichment step.
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