Autoradiographic detection of RNA and glycoprotein synthesis in epithelial cells of the oviduct in ovulated heifers
1999
Pivko, J. | Grafenau, P. | Uhrin, V. | Kopecny, V. (Vyskumny Ustav Zivocisnej Vyroby, Nitra (Slovak Republic))
Heifers (n=9) of the Black-Pied Holstein-Friesian breed were slaughtered on the 3rd, 6th and 9th day of the synchronized cycle, respectively (heat=day 0). Tissue samples from the ampullar part of the oviduct were collected immediately after slaughter and treated for histoautoradiographic (ARG) analyses. Investigated autoradiograms revealed an intensive synthesis of RNA in both cilliated as well as in secretory cells of the oviduct epithelium. The intensity of RNA synthesis remains comparable during the early luteal phase from the 3rd to the 9th day of the cycle. The glycoproteins, synthesized first in the Golgi apparatus in the supranuclear region, were seen to move to the apical region of the epithelial cells later on. The most intensive autoradiographic reaction of newly synthesized glycoproteins was detected on the 3rd day of the cycle without an evident change on the 6th day but decreasing on the 9th day.
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