Morphological changes in transgenic Populus carrying the rolC gene from Agrobacterium rhizogenes
1996
Fladung, M. | Muhs, H.J. | Ahuja, M.R.
We have employed the reporter gene rolC gene from Agrobacterium rhizogenes as a morphologically detectable marker system for investigating growth alterations in Populus. A hybrid aspen (P. tremula L. x P. tremuloides MICHX.) clone, Esch5, was transformed using different chimeric gene constructs including the rolC gene to study its effect on morphological and physiologically-conditioned parameters. Mainly, transgenic aspen carrying the rolC gene under control of the cauliflower-mosaic-virus 35S-promoter and the light inducible rbcS promoter from potato were compared with controls. Other gene constructs, in which rolC expression is prevented by insertion of the transposable element Ac from maize were also included. Differences in growth parameters (e.g. plant height, stem diameter, number of leaves), and growth arrest and terminal bud formation were observed between the control and the 35S- rolC transgenic aspens. Evaluation of onset of dormancy in the autumn and flushing in the next spring revealed differences between untransformed controls and, in particular, the 35S-rolC transgenic plants. These tree- specific morphological and developmental characteristics are discussed in the Light of the transferred foreigngenes in aspen-Populus, a woody plant model system.
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