Ex vitro survival, rooting and initial development of in vitro rooted vs unrooted microshoots from juvenile and mature Tectona grandis genotypes
1997
Bonal, D. | Monteuuis, O.
The influence of in vitro formed adventitious roots on acclimatization and initial ex vitro development of microshoots from juvenile and mature teak (Tectona grandis) genotypes was investigated. Overall, the in vitro rooted microshoots gave rise to higher survival and ex vitro rooting rates 7 weeks after transfer than those not rooted in vitro. The age difference resulted in higher mortality rates 7 and 15 weeks after transfer for the microshoots of mature origin. The number of roots produced in vitro was observed to have a strong influence on the number of roots formed ex vitro 7 weeks after transfer and on the height of the microshoots at the time of transfer, 7 weeks later and to a lesser extent after 15 weeks. Differences in height at transfer between microshoots from the two origins of plant material tended to disappear during the acclimatization process. Overall, more than 80% of the microshoots that were initially transplanted from the various categories tested were successfully acclimatized to ex vitro conditions. These results are discussed considering mainly the influence of the maturation process on the formation of ex vitro roots in in vitro-derived microshoots and emphasizing the role of some basicexogenous factors.
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