Genetic analysis of needle proteins in maritime pine .1. Mapping dominant and codominant protein markers assayed on diploid tissue, in a haploid-based genetic map
1997
Plomion, C. | Costa, P. | Bahrman, N. | Frigerio, J.M.
A genetic analysis of proteins was performed in a 3- generation inbred pedigree of maritime pine (Pinus pinaster (AIT.). Proteins were extracted from needles (2n) and revealed by 2-dimensional gel electrophoresis. A total of 17 qualitative variants (presence/absence variations and position shifts) were observed and conformed to Mendelian inheritance patterns. These markers were localized in a previously reported genetic map based on RAPD markers assayed on megagametophytes (1n). To achieve this integration, evenly spaced RAPD markers were genotyped on diploid tissue. The internal amino acid sequences of 3 proteins were determined, and 2 of them corresponded to genes of known function. We discuss the use of proteins separated in 2-dimensional gels for constructing a genetic map of expressed genes.
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