Changes in starch granules trapped and heated using optical tweezers
1999
Otobe, K. | Kiribuchi-Otobe, C.
A microscopic method to investigate the swelling process of individual starch granules heated in excess water is proposed. A starch granule focused under a light microscope is adjustably trapped using the optical tweezing of a near-infrared laser beam, which simultaneously heats the granule. Using this method, morphological changes in starch granules during heating were continuously recorded and quantitatively analyzed by image-processing techniques. The changes in granules ofdomestic wheat starches with differing amylose content were investigated under irradiation by the laser beam at constant optical power. The shapes of swollen granules of waxy wheat starches were observed to be distinctly different from those of non-waxystarches. Numerical indicators, specifically the time constant of swelling for the granule and its swelling magnification, were also obtained. These showed little relation to the pasting profiles of the same starches, obtained with a Rapid Visco-Analyser.
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