Changes in free amino acids content in Kochia (Kochia scoparia) resistant to ALS-inhibitors
2000
Chodova, D. | Mikulka, J. (Vyzkumny Ustav Rostlinne Vyroby, Prague-Ruzyne (Czech Republic). Odbor Ekologie)
The content of free amino acids was determined in aboveground parts of selected Kochia scoparia populations after treatment with chlorsulfuron and imazapyr. In the population from the locality Kolin (27 % resistant individuals) the total content of free amino acids rose to 155 % and 245 % of the control 24 h after chlorsulfuron treatment (using doses of 22.5 g and 67.5 g/ha), while imazapyr treatment (1000 g/ha) increased the content to 215 % of the control. In populations from the locality Prague-Vrsovice (78 % of resistant individuals) the same treatments resulted in a level of 115 % and 94 % (for chlorsulfuron) and 131 % (for imazapyr) of free amino acids compared to the control. The plants from the two localities differed in the content of the amino acids valine and leucine. A significant decrease of these amino acids in plants from Kolin in contrast to those from Prague-Vrsovice substantiates the opinion of a higher sensitivity of acetolactate synthase (ALS) which is responsible for the biosynthesis of amino acids with branched chains. A significant increase of arginine content was determined in both populations after all herbicide treatments.
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