Micropropagation of Lagerstroemia parviflora through axillary bud culture
1997
Qurajshi, A. | Koche, V. | Mishra, S.K.
Axillary bud cultures were initiated from 3 types of nodal explants of Lagerstroemia parviflora. The cultures derived from explants of seedlings, terminal twigs of a 50-year-old tree and basal-sprouts of another 50-year-old tree showed significant variation in responses at establishment, shoot proliferation and rooting stages. All the 3 types of explants exuded phenolic substances from their cut ends. The exudation was checked by suspending them in a solution of 25 mu M PVP 40 and 522.5 mu M citric acid; and by the addition of 100 mu M PVP 40 and 522.5 mu M citric acid in establishment medium. Leaching continued upto rooting stage, therefore, PVP 40 and citric acid were added in MS medium used for successive transfer and rooting of microshoots. Seedling and basal-sprout explants placed on MS medium with 0.44 mu M BA showed maximum shoot lengths 1.45 cm +/- 0.13 and 1.16 cm +/- 0.22, respectively. Tree explants exhibited best axillary shoot elongation (0.8 cm +/- 0.07) onMS medium without plant growth regulators. The cultures derived from seedling and basal-sprout explants could be successfully maintained upto 6th successive transfers, whereas, those derived from tree explants died after 3rd transfer. Microshoots obtained from seedling and basal- sprout explants showed 10% rooting on MS medium supplemented with 4.9 mu M IBA.
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