Sanitary assessment of stone fruits in East Anatolia (Turkey) and characterisation of a Turkish apple chlorotic leaf spot (ACLSV) isolate
1999
Sipahioglu, M.
Field surveys were carried out in the main stone fruit, especially apricot A total number of 1019 samples were tested by ELISA belonging to 75 different cultivars. The following viruses were identified: ACLSV, PDV, PNRSV. A Turkish isolate of ACLSV from a native apricot variety, differed form the known isolates in its biological properties by not infecting Chenopodium quinoa L. The virus was purified successfully and a polyclonal antiserum was produced by immunising a rabbit. Protein preparation of ACLS-EA was separated on 12 per cent SDS-PAGE, than analysed by Western blotting with polyclonal antiserum produced against isolate. A kit ELISA was prepared for double antibody sandwich immunosorbent assay with a titre of 1:500 determined by dilution series test.
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This bibliographic record has been provided by Mediterranean Agronomic Institute of Bari