Characterization of Hungaria Plum Pox Virus isolates
2003
Kolber, M. (Central Service for Plant Protection and Soil Conservation, Budapest (Hungary))Németh, M. | Krizbai, L. | Ember, I.
Characterization of 97 Hungarian PPV isolates was carried out by ELISA and PCR. PCR detected 59 PPV-M isolates, 25 PPV-D isolates and 15 D+M mixed infections. The serological analysis indicated 72 isolates belonging to PPV-M and 25 to PPV-D serotype. There was a reasonably good correlation between the results of ELISA and PCR. However, detection of PPV-D in mixed infections by PCR indicated the greater sensitivity of this method compared to ELISA. Contradictory results of serological and molecular characterization were obtained only in a single case.
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