PCR identification of lactic acid bacteria populations in corn silage inoculated with lyophilised or activated Lactobacillus buchneri.
2023
SANTOS, A. P. M. dos | SANTOS, E. M. | OLIVEIRA, J. S. de | ARAUJO, G. G. L. de | ZANINE, A. de M. | PINHO, R. M. A. | NASCIMENTO, T. V. C. do | PERAZZO, A. F. | FERREIRA, D. de J. | MACEDO, A. J. da S. | SANTOS, F. N. de S. | ANA PAULA MAIA DOS SANTOS, Alagoas State University - Departament of Animal Science, Santana do Ipanema, Alagoas; EDSON MAURO SANTOS, Federal University of Paraíba, Department of Animal Science, Areia, Paraíba; JULIANA SILVA DE OLIVEIRA, Federal University of Paraíba, Department of Animal Science, Areia, Paraíba; GHERMAN GARCIA LEAL DE ARAUJO, CPATSA; ANDERSON DE MOURA ZANINE, Federal University of Maranhão, Department of Animal Science, Chapadinha, Maranhão; RICARDO MARTINS ARAÚJO PINHO, Federal Institute of Education. Science and Technology of Ceara, Crato. Ceará; THIAGO VINICIUS COSTA DO NASCIMENTO, Federal University of Sergipe, Department of Veterinary., Nossa Senhora da Glória, Sergipe; ALEXANDRE FERNANDES PERAZZO, Federal University of Piaui, Department of Planning and Agricultural Policy, Teresina, Piauí; DANIELE DE JESUS FERREIRA, Federal University of Maranhão, Department of Animal Science, Chapadinha, Maranhão; ALBERTO JEFFERSON DA SILVA MACEDO, Federal University of Viçosa, Department of Animal Science, Viçosa, Minas Gerais; FRANCISCO NAYSSON DE SOUSA SANTOS, Federal University of Maranhão, Department of Animal Science, Chapadinha. Maranhão.
This study aimed to evaluate the effect of inoculation with lyophilised and/or activated Lactobacillus buchneri on lactic acid bacteria populations in corn silage. Experimental treatments consisted of corn silage without additives or silage with the inoculants of L. buchneri (1 x 105 cfu/g) applied according to the manufacturer’s recommendations (1 g/tonne fodder) in the forms of the lyophilised inoculant and pre-activated inoculant. Purified isolates from corn silage with and without the inoculant were identified, and 93% of the isolates corresponded to the lactic acid bacteria of the species Lactobacillus plantarum. Among the isolates, no bacteria of the species L. buchneri were detected. The application of lyophilised or activated L. buchneri improved the microbiological profile and reduced ethanol production in corn silage, even without being identified among the isolates captured 70 days after ensilage.
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