Phenotyping soybean plants transformed with rd29A:AtDREB1A for drought tolerance in the greenhouse and field.
2013
ROLLA, A. A. de P. | CARVALHO, J. de F. C. | FUGANTI-PAGLIARINI, R. | ENGELS, C. | RIO, A. do | MARIN, S. R. R. | OLIVEIRA, M. C. N. de | BENEVENTI, M. A. | MARCELINO-GUIMARÃES, F. C. | FARIAS, J. R. B. | NEUMAIER, N. | NAKASHIMA, K. | YAMAGUCHI-SHINOZAKI, K. | NEPOMUCENO, A. L. | AMANDA ALVES DE PAIVA ROLLA, UEL; JOSIRLEY DE FÁTIMA CORRÊA CARVALHO; RENATA FUGANTI-PAGLIARINI; CIBELLE ENGELS, UEL; ALEXANDRE DO RIO; SILVANA REGINA ROCKENBACH MARIN, CNPSO; MARIA CRISTINA NEVES DE OLIVEIRA, CNPSO; MAGDA A. BENEVENTI, UFRGS; FRANCISMAR CORREA MARCELINO, CNPSO; JOSE RENATO BOUCAS FARIAS, CNPSO; NORMAN NEUMAIER, CNPSO; KAZUO NAKASHIMA, JIRCAS; KAZUKO YAMAGUCHI-SHINOZAKI, The University of Tokyo; ALEXANDRE LIMA NEPOMUCENO, SRI.
The development of drought tolerant plants is a high priority because the area suffering from drought is expected to increase in the future due to global warming. One strategy for the development of drought tolerance is to genetically engineer plants with transcription factors (TFs) that regulate the expression of several genes related to abiotic stress defense responses. This work assessed the performance of soybean plants overexpressing the TF DREB1A under drought conditions in the field and in the greenhouse. Drought was simulated in the greenhouse by progressively drying the soil of pot cultures of the P58 and P1142 lines. In the field, the performance of the P58 line and of 09D-0077, a cross between the cultivarsBR16andP58,was evaluated under four different water regimes: irrigation, natural drought (no irrigation) and water stress created using rain-out shelters in the vegetative or reproductive stages. Although the dehydration-responsive element-binding protein (DREB) plants did not outperform the cultivar BR16 in terms of yield, some yield components were increased when drought was introduced during the vegetative stage, such as the number of seeds, the number of pods with seeds and the total number of pods. The greenhouse data suggest that the higher survival rates ofDREB plants are because of lowerwater use due to lower transpiration rates under well watered conditions. Further studies are needed to better characterize the soil and atmospheric conditions under which these plants may outperform the non-transformed parental plants.
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