Phenotypic, genetic and symbiotic characterization of Erythrina velutina rhizobia from Caatinga dry forest.
2018
RODRIGUES, D. R. | SILVA, A. F. da | CAVALCANTI, M. I. P. | ESCOBAR, I. E. C. | FRAIZ, A. C. R. | RIBEIRO, P. R. de A. | FERREIRA NETO, R. A. | FREITAS, A. D. S. de | FERNANDES JUNIOR, P. I. | DALILA RIBEIRO RODRIGUES, UEPB; ALEKSANDRO FERREIRA DA SILVA, Universidade Federal Rural de Pernambuco, Recife, PE; MARIA IDALINE PESSOA CAVALCANTI, Universidade Federal da Paraíba, Areia, PB; INDRA ELENA COSTA ESCOBAR, UNIVASF; ANA CARLA RESENDE FRAIZ, UNIVASF; PAULA ROSE DE ALMEIDA RIBEIRO, Universidade do Estado da Bahia, Juazeiro, BA; REGINALDO ALVES FERREIRA NETO, Universidade Federal de Pernambuco, Recife, PE; ANA DOLORES SANTIAGO DE FREITAS, Universidade Federal Rural de Pernambuco, Recife, PE; PAULO IVAN FERNANDES JUNIOR, CPATSA.
Erythrina velutina (Mulungu) is a legume ree from Caatinga that associates with rhizobia but the diversity and symbiotic ability of mulungu rhizobia are poorly understood. The aim of this study was to characterize mulungu rhizobia from Caatinga. Bacteria were obteined from Serra Talhada and Caruaru in Caatinga under natural regeneration. The bacteria were evaluated to the amplication of nifH and nod C and to metabolic characteristics. Ten selected bacteria identied by 16S rRNA sequences. They were tested in vitro to NaCl and temperature tolerance, auxin production and calcium phosphate solubilization. The symbiotic ability were assessed in an greenhouse experiment. A total of 32 bacteria were obtained and 17 amplied both symbiotic genes. The bacteria showed a high variable metabolic prole. Bradyrhizobium (6), Rhizobium (3) and Paraburkholderia (1) were identied, differing from their geographic origin. The isolates grew up to 45 ◦ C to 0.51 mol L ? 1 of NaCl. Bacteria which produced more auxin in the medium with l -tryptophan and two Rhizobium and one Bradyrhizobium were phosphate solubilizers. All bacteria nodulated and ESA 90 (Rhizobium sp.) plus ESA 96 (Paraburkholderia sp.) were more efcient symbiotically. Diverse and efcient rhizobia inhabit the soils of Caatinga dry forests, with the bacterial differentiation by the sampling sites.
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