Evaluation of a rapid immunodiagnostic test kit for detection of African lyssaviruses from brain material
2009
Markotter, W.(University of Pretoria Department of Microbiology and Plant Pathology) | York, D.(Molecular Diagnostic Services) | Sabeta, C.T.(Onderstepoort Veterinary Institute OIE Rabies Reference Laboratory) | Shumba, W.(Onderstepoort Veterinary Institute OIE Rabies Reference Laboratory) | Zulu, G.(Onderstepoort Veterinary Institute OIE Rabies Reference Laboratory) | Le Roux, K.(Allerton Veterinary Laboratory) | Nel, L.H.(University of Pretoria Department of Microbiology and Plant Pathology)
A rapid immunodiagnostic test kit was evaluated against a selection of isolates of lyssavirus genotypes occurring in Africa. The test was carried out in parallel comparison with the fluorescent antibody test (FAT) and isolates representing previously established phylogenetic groups from each genotype were included. The specificity of the rapid immunodiagnostic test compared favourably with the FAT and was found to detect all representatives of genotypes 1, 2, 3 and 4 in brain samples of either field cases or suckling mouse brain inoculates.
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