α-Glucosidase inhibition and biochemical analysis of extracts of Ziziphus mauritiana young leaves
2018
Zalilawati Mat Rashid | Nurrul Atikah Mohd Nasir | Natasha Aziz
Ziziphus mauritiana is locally known as ‘bidara’ and commanly used as traditional medicine for various ailments and disease treatments. The aims of this study is to evaluate the biochemical composition and -glucosidase inhibition of Z. mauritiana young leaves extracts. The fresh leaves were used to estimate the proximate values. To sellect young leaves, only the newly-expanded leaves at the end of small branch of plant were sampled. The leaves were extracted separately with aqueous and ethanol using ultrasonication-asssisted technique, while crude polysaccharide was yielded from aqueous extract after ethanol precipitation. The ethanol (EE), aqueous (AE) and polysaccharide (PS) extracts were studied for total phenolics content (TPC), total sugars content (TSC), α-glucosidase inhibition and chemical fingerprints using fourier transform infrared spectroscopy (FTIR). Proximate nutritional analysis revealed the presence of high moisture (70.89%) as well as crude proteins (6.92%), crude fibres (6.17%), ash (3.50%), fats (0.46%) and carbohydrates (12.0%). EE contained the most abundance TPC (24.80 mg GAE/g of extract) compared to AE (20.71 mg GAE/g of extract) and PS (3.28 mg GAE/g of extract). Meanwhile, all extracts contained high amount of carbohydrates with values 127.1 to 186.6 g/mL.AE and PS showed stronger α-glucosidase inhibition compared to EA with IC50 values 0.959, 0.962 and 2.152 mg/mL, respectively. Thus, this research could contribute towards providing valuable scientific information to the local communities and for promoting further research on Z. mauritiana young leaves in Malaysia
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