Control of Aspergilus niger in vitro and in vivo by three Iranian essential oils
2018
Fasihi, H. | Noshirvani, N.
The objective of present study was to determine the antifungal activity of thyme (Thymus vulgaris L.), savory (Satureja hortensis L.) and sage (Salvia officinalis L.) essential oils (EOs) at different concentrations (500, 1000 and 1500 ppm) against Apergillus niger in vitroand in vivo (tomato paste) as a natural food preservative. Gas chromatography-mass spectrometry (GC-MS) was used to analyze the chemical composition of the three EOs.Thymol (30.91%), ρ-cymene (14.66%), carvacrol (11.96%) andborneol (11.39%) were the maincomponents in thyme EO. However, carvacrol (31.32%), γ-terpinene (30.03%), thymol (20.56%); and carvacrol (46.11%), camphor (9.77%), α-thujene (9.09%) were the main components in savory and sage EOs, respectively. Furthermore, the results of disc diffusion test and minimum inhibitory concentrations (MICs) showed the highest antifungal activity for thyme, followed by savory and sage EOs. In vivo tests in tomato paste exhibited the greatest antifungal propertiesfor thyme and sage EOs. Sensory evaluation results showed that all tested EOs had negative effects on the organoleptic properties of tomato paste, however application of them in a food (pizza) were mask their high odorous.
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