Comparative genomic analysis and proteolytic properties of Lactobacillus curieae CCTCC M2011381
2020
Chen, Y. | Xie, J. | Sun, J. | Zhou, Z. | Zhang, R. | Li, H. | Wei, D.
Lactobacillus curieae CCTCC M2011381, a novel strain initially isolated from the brine of stinky tofu, acts as a starter in soymilk but not in milk. We investigated how evolution and physiology help L. curieae adapt to the soy environment. To do so, its whole genome was obtained through a large-scale sequencing using Illumina HiSeq 4000 and PacBio RSII. A comparative genome analysis between the strain and other four non-starter dairy strains, namely Lactobacillus brevis ATCC 367, Pediococcus pentosaceus ATCC 25745, L. plantarum WCFS1, and L. reuteri JCM 1112 was carried out based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Cluster of Orthologous Groups (COG) of protein databases. The results indicated that the assembled genome comprised one circular chromosome (2.1 Mb) without a plasmid. The strain was not only phylogenetically close to L. brevis ATCC 367, but also had a close COG distribution to L. brevis ATCC 367. The strain lacked cell envelope proteinase (CEP) which is used for the initiation of casein utilisation, and some oligopeptide transporter systems for the di-, tri-, and tetrapeptides (Dpp system) as detected by rapid annotation using the Subsystem Technology annotation sever. However, it did have an oligopeptide transporter (Opp) system, a peptidolysis system, and an amino acid synthetic pathway. The present work provides insight into the evolution of the L. curieae M2011381 proteolytic system, and its application in the fermentation of soy material.
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