The factor contributing to higher extractable protein content in natural rubber latex glove as determined by ASTM D5712-99 over ASTM D5712-95 and its relation to allergen content
2006
Hasma H. | Dazylah D. | Siti Nor Qamarina M.
Values for glove extractable protein content determined by ASTM D5712-99 were generally higher than corresponding values obtained by ASTM D5712-95. This resulted from the different procedures used in the two assays. Among all the variables investigated, it was ascertained that differences in extraction medium were the main contributing factor. Extraction with 25 mM phosphate-buffered saline, as in ASTM D5712-99, gave higher protein content than extraction with water, as in ASTM D5712-95. The same was true of the allergen values of the extracts.
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