Qualitative and quantitative estimation of indole-3-acetic acid (IAA) in the seeds of Shorea robusta
1999
Bhattachaiya, A. | Das, B. | Saha, P. K.
In India, Shorea robusta (Family: Dipterocarpaceae) is widely distributed forming extensive forests. This economically important tree could not be exploited by foresters due to the short viability period of the seeds. Among the several factors responsible for early induction of non-viability, indole acetic acid (IAA) is one which varied both qualitatively and quantitatively in the seeds at different stages of maturation. It was observed that the amount of IAA in the seeds decreased with maturity. In immature seeds, the content of free and bound IAA were 0.00750 ug g-1 and 0.00146 ug g-1 of dry seeds respectively. In mature viable seeds both free and bound IAA levels declined to 0.00132 ug g-1 and 0.00063 ug g-1 of seed respectively. The non-viable seeds showed bound IAA of 0.00012 ug g-1 of seed but no free IAA. In the wheat coleoptile extension bioassay, considerable growth promoting activity was observed in the case of free IAA but no significant activity was recorded in the case of bound IAA.
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