Antimicrobial and cytotoxic bioprospection of Thymus Vulgaris and Thymol against Salmonella Enterica Serovar Heidelberg isolated in broiler chicken.
2023
SPERANDIO, J. | BASTOS, A. P. A. | BELLAVER, F. A. V. | DAL BELLO, T. C. | KUHNEN, S. | MILLEZI, A. F. | FONSECA, F. M. da | JHONATAN SPERANDIO, Instituto Federal Catarinense/Campus Concórdia | ANA PAULA ALMEIDA BASTOS, CNPSA | FRANCISCO NOE DA FONSECA, GEM | FRANCIANA A VOLPATO BELLAVER, Instituto Federal Catarinense/Campus Concórdia | THAIS CARLA DAL BELLO, Instituto Federal Catarinense/Campus Concórdia | SHIRLEY KUHNEN, Universidade Federal de Santa Catarina | ALESSANDRA FARIAS MILLEZI, Instituto Federal Catarinense/Campus Concórdia.
Abstract: Salmonella enterica serovar Heidelberg (SH), often isolated from broiler chicken samples, damages the entire production chain due to its high resistance in the environment. The search for sustainable disinfectant agents has intensified, focusing on the action of essential oils. This work's objective was to evaluate the in vitro, effect of Thymus vulgaris essential oil (TEO) and thymol in SH isolated from broilers, and the cytotoxicity in animal cells. Four different concentrations of TEO (0.1%, 0.2%, 0.4%, and 0.8%, (v/v)) were used against five SH isolates obtained from broilers and ATCC 8326. Thymol was evaluated at the concentrations of 0.023%, 0.047%, 0.071%, and 0.094% (v/v). In vitro antibacterial assays were performed by quantifying viable planktonic cells in the broth microdilution test. The MTT technique was used to assess the cytotoxicity in IEC-6 intestinal cells and NCTC fibroblasts. IC50 was assessed testing the concentrations of 0.0625%, 0.125%, 0.25%, and 0.5% of TEO and thymol in 24 hours. The bacterial activity was observed from the 0.2% concentration of TEO since no colony-forming units were observed. Thymol in the concentration of 0.094% controlled 83.33% of the bacteria. TEO presented an IC50 of 0.14% and 1.22%, while the IC50 for thymol was 0.068% and 0.001% for the IEC-6 and NCTC cells, respectively. On the other hand, TEO showed low cytotoxicity in fibroblasts and a potential action capacity to eliminate SH strains in vitro.
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