Transcriptome Analysis of Salt Stress in <i>Hibiscus hamabo</i> Sieb. et Zucc Based on Pacbio Full-Length Transcriptome Sequencing
2021
Longjie Ni | Zhiquan Wang | Xiangdong Liu | Shuting Wu | Jianfeng Hua | Yunlong Yin | Huogen Li | Chunsun Gu
<i>Hibiscus hamabo</i> Sieb. et Zucc is an important semi-mangrove plant with great morphological features and strong salt resistance. In this study, by combining single molecule real time and next-generation sequencing technologies, we explored the transcriptomic changes in the roots of salt stressed <i>H</i><i>. hamabo</i>. A total of 94,562 unigenes were obtained by clustering the same isoforms using the PacBio RSII platform, and 2269 differentially expressed genes were obtained under salt stress using the Illumina platform. There were 519 differentially expressed genes co-expressed at each treatment time point under salt stress, and these genes were found to be enriched in ion signal transduction and plant hormone signal transduction. We used <i>Arabidopsis thaliana</i> (L.) Heynh. transformation to confirm the function of the <i>HhWRKY79</i> gene and discovered that overexpression enhanced salt tolerance. The full-length transcripts generated in this study provide a full characterization of the transcriptome of <i>H</i><i>. hamabo</i> and may be useful in mining new salt stress-related genes specific to this species, while facilitating the understanding of the salt tolerance mechanisms.
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