Effect of Hydropriming and Light Quality Treatments on Sprout Growth and Antioxidant Activity in Brassica oleracea var. capitate Seeds
2022
Lee, H.M. | Ji, B.R. | Jang, B.K. | Park, K.T. | Lee, S.Y. | An, K.G. | Cho, J.S.
In this study, we assessed the effect of hydropriming and light quality treatments on the antioxidant activity and bioactive compounds in Brassica oleracea var. capitate sprouts. B. oleracea seeds were soaked in distilled water and treated with different light wavelengths or in complete darkness for 24 h at 20 ± 1℃. Subsequently, the treated seeds were dried for 24 h, sown on sprouting trays, and then incubated for seven days at 25 ± 2℃ under a 16/8 h light/dark photoperiod with white LEDs. The final percent germination of B. oleracea sprouts was 82.3–95.7%. The fresh weight and shoot length increased significantly in all hydropriming and light quality treatments when compared to the control (soaked in water for 24 h in darkness with no drying). We tested different solvents for extracting bioactive compounds and determined their radical scavenging activity and found that 80% ethanol was the best solvent. Hydro-primed sprouts exhibited significant differences in bioactive compounds and antioxidant activity between the different light treatments. In particular, the blue light treatment resulted in a total flavonoid content and DPPH radical scavenging activity (RC50) of 157.2 mg naringin equivalent (NE)·g⁻¹ and 1.27 mg·mL⁻¹, respectively, and was the most effective treatment for increasing bioactive compounds and antioxidant activity. Therefore, the combined germination pretreatments of hydropriming and light quality induced significant changes in bioactive compounds and antioxidant activity of B. oleracea sprouts.
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