An In Vitro Method to Estimate Apparent Ileal Crude Protein Digestibility in Feed Ingredients Fed to Broiler Chickens
2022
An, S.H. | Kong, C.S.
The objective of this study was to evaluate an in vitro procedure to estimate the crude protein (CP) digestibility of feed ingredients and mixed diets in broiler chickens. The apparent ileal digestibility (AID) of the CP was measured using 23-d-old male broilers. Three experimental diets, containing three feed ingredients, namely soybean meal (SBM), canola meal (CM), and corn distiller’s dried grains with solubles (DDGS), were used as the sole source of CP. A 2-step in vitro procedure was used to estimate in vivo CP digestibility; all the experiments were performed in triplicate. In step 1, the feed ingredient and mixed diet samples were incubated for 4 h at 40℃ with a pH 2.0 pepsin solution, and in step 2, the flasks were incubated for 12 h at 40℃ with a pH 6.8 pancreatin solution. Following incubation, all the samples were filtered; the undigested residues were collected and pooled together to analyze the undigested CP concentration. The in vitro CP digestibility of mixed diets and feed ingredients were 93.2% and 93.0% for SBM, 86.8% and 86.7% for CM, and 83.8% and 79.1% for DDGS, respectively. The coefficients of determination (R²) between the CP digestibility values for the feed ingredients and the in vitro CP digestibility values for the feed ingredients or respective mixed diets were 0.87 or 0.67. The results of the study demonstrated that the in vitro CP digestibility values obtained from the respective mixed diets were better estimates than the values obtained from the individual feed ingredients to predict the AID of CP in feed ingredients fed to broiler chickens.
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