Methods of estimating the half-life of biological activity of toxic chemicals in water
1972
Marking, Leif L.
In the absence of analytical methods, the half-life of biological activity of a chemical can be estimated by bioassays. The methods presented require the determination of the LC50 values (concentration providing 50% mortality) for organisms in aged solutions containing unknown residual concentrations, and concurrent tests of solutions containing known concentrations. The half-life of biological activity is determined by plotting (1) the percent concentrations remaining in aged solutions or (2) the deactivation indices against aging time on cyclic semilogarithmic graph paper. These methods could be used also to determine the half-life of antimycin at different pH's, temperatures, and light intensities, the factors thought to influence the toxicant's efficacy. The methods are not limited to fish and fish toxicants.
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