Comparison of methods for isolation of protozoa from ruminal fluid
1996
Neill, L. | Ivan, M.
Isolation of protozoa from ruminal fluid taken from the same cow on five different days was carried out by three methods based on sedimentation or on filtration using 10-micrometer mesh (PMB-10) or 20-micrometer mesh (PMB-20) polyester monofilament bags. Differences among means for percentage recoveries of protozoal numbers for the three isolation methods were statistically significant (P = 0.007), but those for dry matter and diaminopimelic acid (DAPA) recoveries were not (P > 0.1). The sedimentation method produced the lowest recovery of protozoa as well as the highest recovery of DAPA, which indicated the highest bacterial contamination. Among the filtration-based methods PMB-10 produced higher recoveries of protozoal numbers and dry matter than PMB-20, but with somewhat larger bacterial contamination. Among the three methods compared, filtration with PMB-10 was considered as the most suitable.
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