Highly effective cell synchronization in plant roots by hydroxyurea and amiprophos-methyl or colchicine
1993
Pan, W.H. | Houben, A. | Schlegel, R.
Effective somatic cell synchronization in root-tip meristems and improved chromosome spreading were achieved in white campion, wheat, rye, and barley by application of hydroxyurea and amiprophos-methyl or colchicine, combined with a pretreatment of ice water and modified fixative, as well as enzymatic digestion of the meristems. The protocol provides metaphase indices of approximately 50%. The chromosomes and chromosomal DNA were with minimum distortion, providing useful material for chromosome banding studies, in situ DNA-DNA hybridization, microdissection, and microcloning.
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