Effect of light irradiance on the production of sulfolipids from Anabaena 7120 in a fed-batch photobioreactor
1997
Archer, S.D. | McDonald, K.A. | Jackman, A.P.
Sulfolipids have recently emerged as promising antiHIV and anti-tumor therapeutics. These lipids have been found in association with the photosynthetic apparatus in most photoautotrophic organisms. To date there have been no quantitative studies on the effect of environmental factors on the production of sulfolipid. In this study, we present results on the effect of light irradiance on the production of sulfolipids using the cyanobacterium Anabaena 7120. The cyanobacteria are grown in a 2 L fed-batch photobioreactor at various external-light intensities. Total lipids are extracted using the Folsch procedure and sulfolipids are quantified using thin-layer chromatography and scanning densitometry. We have achieved a maximum of 14 mg sulfolipid/g dry weight of cell. Our results indicate that there are two stages in the specific rate of production of sulfolipids, one in the declining exponential-growth phase of the cells and the other in the light-limited stage of growth.
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