Dimethyl formamide‐free, urea‐NaCl fluorescence in situ hybridization assay for Staphylococcus aureus
2012
Lawson, T.S. | Connally, R.E. | Vemulpad, S. | Piper, J.A.
Aims: To test the feasibility of identifying Staphylococcus aureus with a fluorescence in situ hybridization (FISH) assay that uses a single hot‐plate and urea‐NaCl reagents. Methods and Results: Slides spotted with S. aureus and treated with methanol and lysozyme were incubated with urea‐NaCl reagents on a hot‐plate with a precise temperature control and identified with specific DNA probes. Conclusions: Staphylococcus aureus was detected and differentiated from Staphylococcus epidermidis in 1 h with a novel FISH method that used a single hot‐plate and in the absence of dimethyl formamide. Significance and Impact of Study: A rapid hot‐plate FISH assay with urea‐NaCl and without toxic dimethyl formamide might be useful if FISH is run infrequently or where resources are limited.
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