The use of real-time ultrasonography to select embryo donors participating in a transgenesis goat programme
2008
Teixeira, D.I.A. | Lopes-Júnior, E.S. | Sousa, F.C. | Pinheiro, E.S.P. | Serova, I.A. | Andreeva, L.E. | Freitas, V.J.F.
The efficiency of the transrectal real-time ultrasonography (RTU) scanning technique to monitor the number of corpora haemorraghica (CH) of goat embryo donors was compared to results obtained by a reference method (laparotomy). The ovaries of 24 Saanen goats were observed by RTU, approximately 72h after superovulatory treatment, followed by laparotomy. The diameter of the four largest CHs was measured using image analysis software. The use of ultrasonographic scanning allowed in determining if a goat had ovulated or not in all the females. In all the ovaries examined the total number of CHs counted was lower (p <0.01) with RTU than following the laparotomy method. The correlation coefficient between the number of identified CHs detected by the two methods was significantly positive (r ² =0.71; p <0.001). The sensitivity, specificity and total efficiency of the ultrasonographic observation to determine the occurrence of ovulations in a goat was 100.0%. However, the sensitivity to determine the presence of multiple ovulations was only 25.0%, but the specificity remained 100.0%. The mean diameter of the four largest CHs per ovary following superovulation was 6.88±0.22mm. In conclusion, assessment of the ovulatory response by transrectal RTU can be used to select responding goat donors in a transgenesis programme or to evaluate a donor with multiple ovulations, but not to establish the exact number of CHs.
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