Isolation of amaranth starch by diluted alkaline-protease treatment
1998
Radosavljevic, M. | Jane, J. | Johnson, L.A.
Starch was isolated from Amaranthus cruentus seeds by different alkaline treatments and combinations of low alkaline steeping and protease treatments. For low alkaline-protease treatments, amaranth seeds were steeped in a NaOH solution (0.05%, pH 12) for 22 hr to loosen the protein matrix and ground. The pH of the ground slurry was adjusted to 7.5 and subjected to a protease (from Aspergillus sojae) treatment. The slurry was incubated with 1 or 0.5% of the protease (based on total amount of seeds) for 2 hr at 37 degrees C and 50 rpm. The starch was then isolated by screening and centrifugation. This method produced starch with a low protein content (less than or equal to 0.2%) and a high recovery (approximately 80%). Amaranth starch isolated by alkaline treatments were also studied by using various concentrations of NaOH steeping solutions and with or without alkaline solution during grinding and washing. The properties of amaranth starch isolated by alkaline and low alkaline-protease treatments were analyzed and compared. The properties of the amaranth starch were also compared with those of normal and waxy maize starches.
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