Comparison of teliospore proteins of the two smut fungi Tilletia caries and Tilletia controversa, by SDS electrophoresis
2013
Halász, Á | Szamos, J. | Virányi, F.
The aim of this study was to develop an accurate, fast and safe routine diagnostic method based on protein studies for differentiating between T. caries and T. controversa at species level. Since import of wheat contaminated with T. controversa is restricted by several countries, differentiation of T. controversa from the more prevalent T. caries is of economic interest. The newly developed method is based on distilled water washing followed by the rupturing of the teliospore walls in PBS extraction medium, and an SDS electrophoresis (10% resolving gel). The electrophoretic pattern showed consistent species-related differences in a 106 kDa polypeptide that appeared in each extract of T. controversa, but was not present in the protein extracts of T. caries. The newly developed method could be of value for the authorities performing routine monitoring of T. controversa as an up-to-date diagnostic assay in wheat shipments.
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