Novel immunochromatographic strip assay based on up-conversion nanoparticles for sensitive detection of imidacloprid in agricultural and environmental samples
2021
Lu, Xinying | Chen, Yang | Zou, Rubing | Si, Fangfang | Zhang, Mingzhou | Zhao, Ying | Zhu, Guonian | Guo, Yirong
Neonicotinoid insecticides are widely used in agriculture for pest control, but the pesticide residues in environmental and agricultural products were a big threat to the health of non-target organisms. In this study, a new immunochromatographic strip test was established for the rapid detection of imidacloprid residue, a neonicotinoid insecticide, based on up-conversion nanoparticles (UCNPs) coupled with the monoclonal antibody against imidacloprid. Under optimal conditions, the half inhibitory concentration (IC₅₀), detection limit, and the linear range of this strip were 8.37 ng/mL, 0.45 ng/mL, and 0.97–250 ng/mL. The strip test could be completed in 30 min. The average recoveries of imidacloprid spiked in water, Chinese cabbages, cucumber, honey, and tea samples were 70.1~101.8%, with coefficient of variations less than 18.9%. The strip was used to test real samples and verified by UPLC-MS/MS method with the good agreement (R² was 0.9825), indicating this novel strip immunoassay is accurate and reliable.
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