Mapping of endoglucanases displayed on yeast cell surface using atomic force microscopy
2017
Takenaka, Musashi | Kobayashi, Takuya | Inokuma, Kentaro | Hasunuma, Tomohisa | Maruyama, Tatsuo | Ogino, Chiaki | Kondo, Akihiko
The surface of yeast cells has been an attractive interface for the effective use of cellulose. Surface enzymes, however, are difficult to visualize and evaluate. In this study, two kinds of unique anchoring regions were used to display the cellulase, endoglucanase (EG), on a yeast cell surface. Differences in the display level and the localization of EG were observed by atomic force microscopy. By surveying the yeast cell surface with a chemically modified cantilever, the interactive force between the cellulose and EG was measured. Force curve mapping revealed differences in the display levels and the localization of EG according to anchoring regions. The proposed methodology enables visualization of displayed enzymes such as EG on the yeast cell surface.
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