An ultrasensitive chemiluminescence immunoassay for fumonisin B1 detection in cereals based on gold‐coated magnetic nanoparticles
2018
Jie, Mingsha | Yu, Songcheng | Yu, Fei | Liu, Lie | He, Leiliang | Li, Yanqiang | Zhang, Hongquan | Qu, Lingbo | Harrington, Peter de B | Wu, Yongjun
BACKGROUND: In the present study, a novel highly sensitive magnetic enzyme chemiluminescence immunoassay (MECLIA) was developed to detect fumonisin B₁ (FB₁) in cereal samples. The gold‐coated magnetic nanoparticles (Fe₃O₄@Au, GoldMag) were used as solid phase carrier to develop a competitive CLIA for detecting FB₁, in which FB₁ in samples would compete with FB₁‐ovalbumin coated on the surface of Fe₃O₄@Au nanoparticles for binding with FB₁ antibodies. Successively, horseradish peroxidase labeled goat anti‐rabbit IgG (HRP‐IgG) was conjugated with FB₁ antibodies on the microplate. In substrate solution containing luminol and H₂O₂, HRP‐IgG catalyzed luminol oxidation by H₂O₂, generating a high chemiluminescence signal. The FB₁ immune GoldMag particles were characterized by Fourier transform infrared spectroscopy, scanning electron microscope and zeta potential analysis, etc. RESULTS: The concentrations and the reaction times of these immunoreagents were optimized to improve the performances of this method. The established method could detect as low as 0.027 ng mL–¹ FB₁ from 0.05 ng mL–¹ to 25 ng mL–¹, demonstrating little cross‐reaction (less than 2.4%) with other structurally related compounds. The average intrassay relative SD (RSD) (n = 6) was 3.4% and the average interassay RSD (n = 6) was 5.4%. This method was successfully applied for the determination of FB₁ in corn and wheat and gave recoveries of between 98–110% and 91–105%, respectively. CONCLUSION: The results of the present study suggest that the MECLIA approach has potential application for high‐throughput fumonisin screening in cereals. © 2018 Society of Chemical Industry
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