Efficacy of various collection methods for oocyte retrieval in buffalo
1996
Das, G.K. | Jain, G.C. | Solanki, V.S. | Tripathi, V.N.
The efficacy of 3 oocyte collection methods and the effect of the presence of a CL on oocyte recovery from buffalo ovaries obtained at an abattoir were assessed. Oocytes were collected slicing (n=131), follicle puncture (n=86) and follicle aspiration (n=80). Slicing yielded significantly (P<0.01) more (5.7) oocytes per ovary than follicle puncture (2.6) or aspiration (1.7). Better quality oocytes (good and fair)were recovered per ovary by slicing (2.6) than by puncture (1.3) or aspiration (0.9). However, follicle puncture took the least time (1.5 min) compared with aspiration (1.6 min) and slicing (2.8 min). The presence of a CL significantly (P<0.05) reduced the recovery rate as well as the quality of the oocytes. Slicing was found to be the overall best oocyte collection method among the 3 tested in this study.
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