Analysis of differentially methylated regions of genomic DNA in maize (Zea mays L.) exposed to salt stress
2018
He, Xuechuan | Xie, Yonghong | Zhao, Yinchuan | Gao, Xinyan | Yu, Lulu | Ding, Ying | Wu, Qian | Wang, Qian | Jiang, Duo | Song, Xianliang | Li, Ping | Wang, Baohua
Through the analysis of DNA methylation in a maize inbred line under salt stress, we investigated the molecular mechanism of salt tolerance in maize. The methylation sensitive amplification polymorphism (MSAP) combined with capillary electrophoresis was applied to detect DNA methylation, and some differentially methylated DNA sequences were cloned and analyzed to discover interesting genes which might contribute to maize salt tolerance. The maize inbred line LH196 experienced extensive DNA methylation variations, including hypo- and hyper-methylation and changes of DNA methylation types. Cloning, sequencing and real-time quantitative PCR tests found that a variety of related genes play important roles in maize salt tolerance.
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