Ethylene production by Agrobacterium rhizogenes strains in vitro and in vivo
2003
Kepczynska, E. | Zielinska, S. | Kepczynski, J.
Research was initiated to determine whether Agrobacterium rhizogenes strains, used for plant transformation, are a source of ethylene and which compound these bacteria use for its production. A4 and LBA strains produced ethylene on solid MG medium, used for culture of bacteria, and on MS medium used for plant in vitro culture. The enhanced ethylene production in the presence of methionine and 2-keto-4-methylthiobutyric acid (KMBA), but not in the presence of glutamic acid and 1-aminocyclopropano-1-carboxylic acid (ACC), was observed. The removing of ethylene from the culture atmosphere caused the inhibition of bacterial growth and indicates, that these strains need this gas for their growth. The inoculation of petunia explants with A. rhizogenes caused increased ethylene production by the explants. Ethylene present in flasks during the growth of hairy roots of Petunia hybrida inhibited their growth. These results indicate that in the flasks where the plant transformation takes place the source of ethylene, affecting the root growth, is possibly not only the plant explant but also bacteria and pathogenesis.
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