Propagation and conservation of Lilium polyphyllum D. Don ex Royle
2014
Dhyani, Anurag | Sharma, Gunjan | Nauṭiyāla, Bhagavatī Prasāda | Nautiyal, Mohan Chandra
In the present study conventional and in vitro propagation protocols have been developed for Lilium polyphyllum D. Don ex Royle. Sodium hypochlorite (4%) treatment improves seed germination (82±7.64%). For vegetative propagation, IBA 100ppm treatment produced 3±1.07 bulblets/scale. We achieved multi-bulblet formation through in vitro seed germination and callus culture using bulb scales on MS medium. The study reports 91% in vitro germination using chipped seed, treated with IBA 100ppm within 14–18 days of inoculation. This is the first report of germination >90% within <20 days in the genus Lilium having hypogeal germination. After 18 days of germination, the in vitro seedlings showed multi-bulblet structures. Bulb scales maximum callusing (69±6.94%) and bulblet formation (1.7±0.18 bulblets/scale) were observed in MS medium supplemented with IBA 25μM. Bulblet formation was observed after four weeks following two sub-cultures on the same medium. Later individual bulblets were hardened with >40% survival after a year. The study can serve as a tool of mass multiplication for in situ and ex situ conservation of the species.
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