MicroRNA-1228* inhibit apoptosis in A549 cells exposed to fine particulate matter
2016
Li, Xiaobo | Ding, Zhen | Zhang, Chengcheng | Zhang, Xin | Meng, Qingtao | Wu, Shenshen | Wang, Shizhi | Yin, Lihong | Pu, Yuepu | Chen, Rui
Studies have reported associations between fine particulate matter (PM₂.₅) and respiratory disorders; however, the underlying mechanism is not completely clear owing to the complex components of PM₂.₅. microRNAs (miRNAs) demonstrate tremendous regulation to target genes, which are sensitive to exogenous stimulation, and facilitate the integrative understood of biological responses. Here, significantly modulated miRNA were profiled by miRNA microarray, coupled with bioinformatic analysis; the potential biological function of modulated miRNA were predicted and subsequently validated by cell-based assays. Downregulation of miR-1228-5p (miR-1228*) expression in human A549 cells were associated with PM₂.₅-induced cellular apoptosis through a mitochondria-dependent pathway. Further, overexpression of miR-1228* rescued the cellular damages induced by PM₂.₅. Thus, our results demonstrate that PM₂.₅-induced A549 apoptosis is initiated by mitochondrial dysfunction and miR-1228* could protect A549 cells against apoptosis. The involved pathways and target genes might be used for future mechanistic studies.
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